Modelo de negocios para la creación de un restaurante especializado en Fondue

El objetivo del presente trabajo es dar a conocer la factibilidad económica y financiera para la creación de un restaurante especializado en fondue en la ciudad de Pereira. Gracias a los objetivos específicos se pudo analizar el mercado y conocer la factibilidad de la creación del restaurante desde el punto de vista técnico, económico y financiero. El presente estudio se realizó en base en una investigación de campo, los datos se recogieron por medio de la técnica de encuestas y el instrumento fueron cuestionarios físicos y virtuales que se realizaron a los habitantes de la ciudad El estudio comprende en primer lugar las bases y la conceptualización del proyecto definiendo objetivos claros y concisos a alcanzar, por otra parte se realiza un diagnóstico de la oferta y demanda por medio de un estudio de mercados obteniendo como resultados los requerimientos y expectativas que tiene los habitantes de la ciudad con respecto al proyecto, de otro lado, se define la localización por medio de la calificación por puntos y asimismo se realiza el estudio de tamaño en el que se define la capacidad instalada; igualmente se define la ingeniería del producto determinado todas las especificaciones del mismo analizando todo lo concerniente con la parte misional del restaurante, además se estructura toda la parte administrativa fijando el direccionamiento estratégico de la empresa y sus respectivos trámites legales, de igual modo se procede a definir las inversiones que se deben realizar para estructurar el restaurante determinando los activos y la capacidad de trabajo y por consiguiente su financiamiento, también dentro de la estructura del estudio de factibilidad se determinan los presupuestos de ingresos y costos en el que se elaboran estados financieros como el balance general y el estado de pérdidas y ganancias, finalmente se procede a realizar la evaluación para verificar la viabilidad del proyecto por medio del estudio de financiamiento el cual se obtiene a través de la tasa interna de retorno (TIR) y por último se desarrolla la evaluación socio- económica. A través del estudio se visualizaran diferentes análisis e investigaciones las cuales arrojaran el nivel de pertinencia y viabilidad para estructurar el restaurante La Fondue en la ciudad de Pereira, siendo este un proyecto beneficioso y aportante para dicha zona

Association of beta 1 integrin with focal adhesion kinase and paxillin in differentiating Schwann cells

Schwann cells (SCs) differentiate into a myelinating cell when simultaneously adhering to an axon destined for myelination and basal lamina. We are interested in defining the signaling pathway activated by basal lamina. Using SC/sensory neuron (N) cocultures, we identified beta 1 integrin and F-actin as components of a pathway leading to myelin gene expression and myelination (Fernandez-Valle et al., 1994, 1997). Here, we show that focal adhesion kinase (FAK) and paxillin are constitutively expressed by SCs contacting axons in the absence of basal lamina. Tyrosine phosphorylation of FAK and paxillin increases as SCs form basal lamina and differentiate. FAK and paxillin specifically coimmunoprecipitate with beta 1 integrin in differentiating SC/N cocultures but not SC-only cultures. Paxillin coimmunoprecipitates with FAK and fyn kinase in differentiating SC/N cocultures. A subset of tyrosine-phosphorylated b1 integrin, FAK, and paxillin molecules reside in the insoluble, F-actin-rich fraction of differentiating cocultures. Cytochalasin D, an actin depolymerizing agent, decreases tyrosine phosphorylation of FAK and paxillin and their association with b1 integrin and causes a dose-dependent increase in the abundance of insoluble FAK and paxillin complexes. Collectively, our work indicates that b1 integrin, FAK, paxillin, and fyn kinase form an actin-associated complex in SCs adhering to basal lamina in the presence of axons. This complex may be important for initiating the process of SC differentiation into a myelinating cell

Bacteria isolated from Bengal cat (Felis catus × Prionailurus bengalensis) anal sac secretions produce volatile compounds potentially associated with animal signaling.

In social animals, scent secretions and marking behaviors play critical roles in communication, including intraspecific signals, such as identifying individuals and group membership, as well as interspecific signaling. Anal sacs are an important odor producing organ found across the carnivorans (species in the mammalian Order Carnivora). Secretions from the anal sac may be used as chemical signals by animals for behaviors ranging from defense to species recognition to signaling reproductive status. In addition, a recent study suggests that domestic cats utilize short-chain free fatty acids in anal sac secretions for individual recognition. The fermentation hypothesis is the idea that symbiotic microorganisms living in association with animals contribute to odor profiles used in chemical communication and that variation in these chemical signals reflects variation in the microbial community. Here we examine the fermentation hypothesis by characterizing volatile organic compounds (VOC) and bacteria isolated from anal sac secretions collected from a male Bengal cat (Felis catus × Prionailurus bengalensis), a cross between the domestic cat and the leopard cat. Both left and right anal sacs of a male Bengal cat were manually expressed (emptied) and collected. Half of the material was used to culture bacteria or to extract bacterial DNA and the other half was used for VOC analysis. DNA was extracted from the anal sac secretions and used for a 16S rRNA gene PCR amplification and sequencing based characterization of the microbial community. Additionally, some of the material was plated out in order to isolate bacterial colonies. Three taxa (Bacteroides fragilis, Tessaracoccus, and Finegoldia magna) were relatively abundant in the 16S rRNA gene sequence data and also isolated by culturing. Using Solid Phase Microextraction (SPME) gas chromatography-mass spectrometry (GC-MS), we tentatively identified 52 compounds from the Bengal cat anal sac secretions and 67 compounds from cultures of the three bacterial isolates chosen for further analysis. Among 67 compounds tentatively identified from bacterial isolates, 51 were also found in the anal sac secretion. We show that the bacterial community in the anal sac consists primarily of only a few abundant taxa and that isolates of these taxa produce numerous volatiles that are found in the combined anal sac volatile profile. Several of these volatiles are found in anal sac secretions from other carnivorans, and are also associated with known bacterial biosynthesis pathways. This is consistent with the fermentation hypothesis and the possibility that the anal sac is maintained at least in part to house bacteria that produce volatiles for the host

Amlexanox Enhances Premature Termination Codon Read-Through in COL7A1 and Expression of Full Length Type VII Collagen: Potential Therapy for Recessive Dystrophic Epidermolysis Bullosa.

Recessive dystrophic epidermolysis bullosa (RDEB) is a rare monogenic blistering disorder caused by the lack of functional type VII collagen, leading to skin fragility and subsequent trauma-induced separation of the epidermis from the underlying dermis. A total of 46% of patients with RDEB harbor at least one premature termination codon (PTC) mutation in COL7A1, and previous studies have shown that aminoglycosides are able to overcome RDEB PTC mutations by inducing read-through and incorporation of an amino acid at the PTC site. However, aminoglycoside toxicity will likely prevent widespread clinical application. Here the FDA-approved drug amlexanox was tested for its ability to read-through PTC mutations in cells derived from patients with RDEB. Eight of 12 different PTC alleles responded to treatment and produced full length protein, in some cases more than 50% relative to normal controls. Read-through type VII collagen was readily detectable in cell culture media and also localized to the dermal-epidermal junction in organotypic skin culture. Amlexanox increased COL7A1 transcript and the phosphorylation of UPF-1, an RNA helicase associated with nonsense-mediated mRNA decay, suggesting that amlexanox inhibits nonsense-mediated mRNA decay in cells from patients with RDEB that respond to read-through treatment. This preclinical study demonstrates the potential of repurposing amlexanox for the treatment of patients with RDEB harboring PTC mutation in COL7A1

Study of shape memory effect in stainless steel using compression tests

The shape recovery properties of an Fe-Mn-Si-Cr-Ni-Co based alloy were studied using compression tests. Analyzed were the elastic recovery (Er), shape recovery (Sr) and total shape recovery (T SR = Er + Sr) measurements as a function of training cycles. The results indicated that 3.3 was the best ratio between height (h o = 20 mm) and diameter (φo = 6 mm) to obtain a homogeneous deformation and defined loading curves. A major contribution of T SR was attributed to shape recovery. In the last training cycle, a T SR = 90 % was obtained, where 25% was attributed to Er.As propriedades de recuperação de forma da liga à base de Fe-Mn-Si-Cr-Ni-Co foram estudadas usando ensaio de compressão. Foram analisadas medidas de recuperação elástica (Re), recuperação de forma (Rf) e recuperação de forma total (R T = Re + Rf) em função do número de ciclos de treinamento. Os resultados indicaram que 3,3 foi a melhor razão entre altura (h o = 20 mm) e diâmetro (φo = 6 mm) para se conseguirem uma deformação homogênea e curvas de carregamento bem definidas. A maior contribuição para a R T foi atribuída à recuperação de forma. No último ciclo de treinamento, foi obtida uma R T = 90%, sendo 25% atribuída a Re.493499Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Determination of the mechanical properties of epsilon martensite by nanoindentation in shape memory stainless alloys

ABSTRACT This work presents a hardness study and elastic modulus for the ε-martensite and γ-austenite phases of an iron based shape memory alloy. Using instrumented indentation, it was possible to determine the hardness and elasticity modulus of these phases separately. The martensitic phase presented a hardness of 7.0 GPa and the austenitic phase presented a hardness of 3.0 GPa. The elastic modulus values were 202 and 137 GPa for the martensitic and austenitic phases, respectively.RESUMO Esse trabalho apresenta um estudo da dureza e do mee austenita-γ da liga inoxidável à base de ferro com efeito de memória de forma. Utilizando indentação instrumentada, foi possível determinar a dureza e o módulo de elasticidade das fases austenita-γ e martensita-ε separadamente. A fase martensítica apresentou uma dureza de 7,0 GPa, enquanto que a dureza da fase austenítica foi de 3,0 GPa. Os valores de módulo de elasticidade foram 202 e 137 GPa, para as fases martensita e austenita, respectivamente.3944Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Emerging issues and key focus areas in food science, technology, and engineering higher education

Food studies is an interdisciplinary field involving food production, distribution, consumption, and social, economic, and environmental impacts. Food science, technology, and engineering have undergone a significant transformation over the past few decades. Moreover, the food industry faces numerous critical challenges ranging from food safety, sustainability, health, and nutrition to changing consumer preferences and global food security. The food industry needs a workforce prepared for the most recent technical developments and also highly skilled. Working group 1.2, on Emerging Issued, Key Focus Areas, of IUFoST (International Union of Food Science and Technology) activities address education materials and methods on topics such as sustainability of food systems, emerging technologies, food safety, food insecurity, ethical considerations, among others. The working group organizes webinars series on teaching methods and methodologies, current critical topics in food studies, and, more recently, is opening an international web forum. This forum will promote effective communication and sharing of information in the field. It will cover topics such as curricula development, education in specific world regions, opportunities of collaboration, research education-related topics, and industry collaborations. Working group 1.2 of IUFoST is also a key element for networking with several other organizations working towards a new generation of food science, technology, and engineering professionals with the skills and knowledge required to drive innovation and reshape the industry.info:eu-repo/semantics/publishedVersio

Inhibition of tropomyosine receptor kinase B on the migration of human Schwann cell and dispersion of oral tongue squamous cell carcinoma in vitro

Background Schwann cells (SC) may play an important role in perineural invasion (PNI) by promoting cancer cell dispersion. Brain‐derived neurotrophic factor (BDNF) may contribute to these cellular events by activating tropomyosine receptor kinase B (TrkB). This study examines the effect of TrkB inhibition on SC migration and oral cancer cell dispersion in vitro. Methods Human tongue squamous cell carcinoma (SCC‐9) and human SCs were cocultured in three different conditioned mediums: control, BDNF, and TrkB inhibitor. Cell migration, cancer cell dispersion, and SC dedifferentiation were measured on time‐lapse and immunofluorescence images. Results Cancer cell migration exceeded SC migration in all conditions. TrkB inhibition promoted SC dedifferentiation and significantly increased SC migration, when compared to BDNF conditions. TrkB inhibition also reduced cancer cell dispersion, when compared to control and BDNF‐treated cultures. Conclusion SCs may have importance in the pathophysiology of PNI. TrkB inhibition may be a potential avenue for therapeutic intervention